Session: Clemens-Winkler & Gerhard-Hesse Award Session

Session Chair: Dr. Martin Vogel

Introduction (Dr. Martin Vogel)

22-Jun-2022 15:00 (5 Minutes) ICM/Hall 4b

2021 Gerhard-Hesse Award to Dr. Katja Dettmer-Wilde, Laudation Prof. Dr. Frank-Michael Matysik, Award Lecture: Metabolomics from an analytical chemistry perspective

Dr. Katja Dettmer-Wilde, Uni Regensburg
Pronounced changes in metabolism are a well-recognized trait of many pathophysiological processes. Due to the complexity of the metabolome, a combination of different analytical platforms is essential to comprehensively describe these metabolic alterations. Mass spectrometry commonly hyphenated to a separation technique such as gas or liquid chromatography is a powerful tool in targeted and untargeted metabolomics providing high detection sensitivity and the ability for structure elucidation of unknowns. A central focus of our work in the field of metabolomics is the development of an array of robust methods to qualitatively and quantitatively probe the metabolome. Selected examples of the application of these methods for example to identify the endogenous lactonization of the oncometabolite D-2-hydroxyglutarate [1] or to study metabolic consequences of pathogen infection will be presented. In addition to analyzing metabolite abundancies, stable isotope labeling experiments are widely used in metabolomics to gain insights into the utilization of metabolic pathways. In this context, data must be corrected for natural stable isotope abundance and tracer purity before data interpretation. IsoCorrectoR a correction tool for MS- and MS/MS-data will be presented [2], and results of selected stable isotope tracing experiments discussed.
22-Jun-2022 15:05 (40 Minutes) ICM/Hall 4b

Multidimensional separation techniques for phospholipid analysis

Prof. Dr. Münster Hayen, WWU Münster
The analysis of lipids is of paramount importance due to their involvement in various biological and physiological processes. A relevant lipid category are phospholipids with a variety of different head groups in combination with fatty acid chains featuring different numbers of carbon atoms and double bonds. The already huge structural diversity is further increased by oxidation. High resolution mass spectrometry (HRMS) is capable of identifying lipid species in combination with fragmentation experiments (MS/MS) based on the accurate m/z and fragmentation pattern. For the differentiation of isomeric lipids or isobaric interferences, however, powerful additional separation techniques are required. Furthermore, oxidation of unsaturated lipids poses a special challenge, as they are usually present in low concentrations and further isomeric species, e.g. hydroperoxylated vs dihydroxylated products. Hence, powerful multidimensional analysis techniques are required for the analysis of the diversity of phospholipids and their oxidation products and showcased in this presentation. The potential of hydrophilic interaction liquid chromatography (HILIC) for class-specific phospholipid separation is demonstrated. Furthermore, HILIC is applied in 2D heart-cut methods as first dimension for the analysis of (oxidized) phospholipids. The obtained fractions are then further investigated by reversed phase liquid chromatography (RPLC) coupled to electrospray-HRMS/MS. The approaches based on two-dimensional heart-cut HPLC-MS are then complemented by ion mobility spectrometry (IMS) combined with HR-MS detection as a powerful tool for efficient and fast analysis of phospholipids and their oxidation products. Mobility-resolved MS/MS experiments are utilized to differentiate isomeric oxidation products. Therefore, by the presented multidimensional separation techniques, the information content and the peak capacity are increased for in-depth phospholipid characterization.
22-Jun-2022 15:45 (30 Minutes) ICM/Hall 4b

2022 Clemens-Winkler Medal to Prof. Dr. Irene Nehls, Laudation Dr. Matthias Koch

22-Jun-2022 16:15 (30 Minutes) ICM/Hall 4b